Projects

MSU Award Number

361463-191001-021000

Current NGI File Number

19-NGI3-78

Associated MSU Award or NGI File Numbers

191001-363513-3K

Start Date

10/1/2019

End Date

2022-9-30 0:0:0

Project Status

Active

Award Amount

$301,329.00

Project Title

Building NOAA ‘Omics and Bioinformatics Capacity for Reproducible and Efficient Analysis of Environmental Samples

PI Last Name

Thompson

PI First Name

Luke

PI Affiliation

MSU

Research Theme

EM-DM

Funding Source

OAR

Project Description

Building ‘omics and bioinformatics capacity for reproducible and efficient analysis of environmental samples requires a data framework with metadata standards and a metadata quality control workflow, a sequence data quality control workflow, expanded amplicon reference sequence databases, and an improved amplicon processing workflow. This project produced a draft OAR 'Omics Data Management Plan to be published as a NOAA Technical Report, data and metadata standards (https://microbiomedata.org/), and a Tourmaline amplicon sequence analysis workflow version 2 (https://github.com/aomlomics/tourmaline). Two different magnetic bead DNA extraction protocols were developed and run on the KingFisher Flex bead-handling robot. PCR preparation, PCR cleanup, DNA dilution, and extraction prep protocols were developed and run on the Opentrons liquid handling robot. The 'omics lab at AOML was modernized, including the integration of the two robots for sample processing.